Hematological and biochemical changes associated with COVID 19

Introduction: Severe acute respiratory syndrome coronavirus causing coronavirus disease 2019 has rapidly evolved from an epidemic outbreak in Wuhan, China, into a pandemic affecting more than 1 million individuals worldwide. It is associated with leucopenia , thrombocytopenia, deranged LFT and elevated inflammatory markers including CRP , D-DIMER , LDH and ferritin Aims and Objectives: 1. To find the proportion of hematopoietic and biochemical changes in category A, B, C patients 2. To find the proportion of inflammatory markers elevated in category C patients. Methods: A cross sectional study among 500 COVID 19 patients who were diagnosed with RT PCR admitted in MES medical college hospital. Routine investigations along with inflammatory markers are analysed. Results: In the hemogram, 38 % had leukocytosis , 8 % had leucopenia, 7 % had thrombocytopenia , 85% had elevated CRP, 61 % had elevated D- dimer, 37 % had elevated Ferritin and 24 % had elevated LDH. Conclusion: Analyzing the inflammato.y markers, hematological and biochemical derangements, and how they correlate with disease severity.

Molecular Dynamic Simulation Search for Possible Amphiphilic Drug Discovery for Covid-19

To determine whether quaternary ammonium (k21) binds to Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) spike protein via computational molecular docking simulations, the crystal structure of the SARS-CoV-2 spike receptor-binding domain complexed with ACE-2 (PDB ID: 6LZG) was downloaded from RCSB PD and prepared using Schrodinger 2019-4. The entry of SARS-CoV-2 inside humans is through lung tissues with a pH of 7.38-7.42. A two-dimensional structure of k-21 was drawn using the 2D-sketcher of Maestro 12.2 and trimmed of C18 alkyl chains from all four arms with the assumption that the core moiety k-21 was without C18. The immunogenic potential of k21/QA was conducted using the C-ImmSim server for a position-specific scoring matrix analyzing the human host immune system response. Therapeutic probability was shown using prediction models with negative and positive control drugs. Negative scores show that the binding of a quaternary ammonium compound with the spike protein's binding site is favorable. The drug molecule has a large Root Mean Square Deviation fluctuation due to the less complex geometry of the drug molecule, which is suggestive of a profound impact on the regular geometry of a viral protein. There is high concentration of Immunoglobulin M/Immunoglobulin G, which is concomitant of virus reduction. The proposed drug formulation based on quaternary ammonium to characterize affinity to the SARS-CoV-2 spike protein using simulation and computational immunological methods has shown promising findings.

SARS-CoV-2 and oral health: Implications and recommendations for dental practice reformation post pandemic

Dental clinics can play a crucial role in spreading the SARS-CoV-2 infections because of the nature of close contact with the patient’s oral cavity and aerosol generating essential dental procedures. Dental practices in regions affected with SARS-CoV-2 are needed to take utmost precautions and follow strict effective infection control protocols even when the movement control/lockdown is gradually lifted post pandemic. Thus, we aim to summarise the current research findings on SARS-CoV-2 in dental settings and relevant international guidelines and recommended management protocols for the dental clinica. The knowledge on the viral properties of SARS-CoV-2 and epidemiologic characteristics are essential to implement proper mitigation strategies against transmission of Covid-19 infections via dental clinics. © 2020, Institute of Medico-Legal Publications. All rights reserved.